|Title:||以Ayerell-Norris法分析Parathion遺毒量P-Nitrophenol移出之必要性||Other Titles:||The Necessity for Removal p-Nitrophenol of Parathion Residue Analysis with Averell-Norris Method||Authors:||梁同庭
|Keywords:||parathion||Issue Date:||Dec-1964||Publisher:||植病保護學會||Journal Volume:||6||Journal Issue:||4||Start page/Pages:||222-224||Source:||植物保護學會會刊||Abstract:||
In some cases, the method of parathion residue analysis developed by Averell and Norris usually detects more residue than is realy present. This is due to the existance of the color which results from the coupling of N-(l-naphthyl)-eth-ylenediamine dihydrochioride with the reduced and diazotizated No2 radical on the p-nitrophenol of parathion. But this radical is still attached to p-nitrophenol and remains in the extract, when the residue is hydrolized and decomposed in alkaline or in natural conditions. Therefore the p-nitrophenol from the extract must be removed before the development of the color.
In this experiment, the material used for removal of p-nitrophenol from parathion residue was 5% sodium bicarbonate solution. When the cleanup was completed, p-nitrophenol would have been extracted twice using sodium bicarbonate solution in a separate funnel in the volume 1/3 of the volume of the extract.
Following this, came a washing using saturated sodiom chloride on volume equal to 34 the volume of extract. All the lower water portions were discarded. Then followed the procedure of analysis. The water remaining must be removed in advance with anhydrous sodium sulfate.
From the results of the experiment, when p-nitrophenol is removed, the residus of parathion estimated by Averell-Norris method will be lower and much closer to the results obtained from bioassay than the results obtained by the method when p-nitrophenol is not removed.
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