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Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/6669
Title: Expression of recombinant Pichia pastoris X33 phytase for dephosphorylation of rice bran fermented liquid
Authors: Ming-Hui Chang 
Chiu-Chung Young
Shiuan-Yuh Chien
A.B. Arun
Keywords: phytase;Pichia pastoris X33;phosphorus;rice bran;SDS-PAGE
Issue Date: Jun-2008
Publisher: Springer
Journal Volume: 58
Journal Issue: 2
Start page/Pages: 233-238
Source: Annals of Microbiology 
Abstract: 
The Aspergillus ficuum phytase gene phyA was overexpressed in Pichia pastoris X33 after replacing Buffered glycerol-complex medium (BMGY medium) using 1% (v/v) glycerol with fresh Buffered methanol-complex medium (BMMY medium) using 1% (v/v) methanol (on daily basis) as carbon sources. The phytase activity increased evidently with the induction time, and reached 200 U mL(-1) after 9 days of induction. We examined the possibility of employing thus obtained phytase to recover phosphorus from the fermented liquid of rice bran. When the 0.1 M sodium acetate buffer was replaced with de-ionised water (pH 5.5 +/- 0.1) as an enzyme reaction solution, there was an increase in the phosphorus recovery with respect to time and reached 1.31% after 24 h incubation contributing to 81% release of inorganic P from the rice bran phytate. Studies on hydrolysis of rice bran phytate by the addition of different concentrations of phytase ranging from 0-200 U mL(-1) produced through the recombinant yeast shows no significant effect in the rate of phytate hydrolysis at enzyme activities of 200, 100, 50 U mL(-1). However, rate of hydrolysis varied significantly at 20, 5, 0 U mL(-1) enzyme concentrations.
URI: https://scholars.tari.gov.tw/handle/123456789/6669
https://link.springer.com/article/10.1007/BF03175322
ISSN: 1590-4261
DOI: 10.1007/BF03175322
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