溶磷菌與高磷需求作物之相容性研究
Other Title
Compatibility of Phosphate Solubilizing Bacteria Toward Crops with High Phosphorus Demand
Start Page
46
End Page
65
ISBN
978-986-5440-51-0
Date Issued
2019-12
Date
2019-12
Author(s)
張鈞喆
賴威安
楊秋忠
沈佛亭
Chun-Tse Chang
Wei-An Lai
Chiu-Chung Yang
Fo-Ting Shen
Abstract
本研究針對菌株是否可在根表面拓殖特性進行探討，篩選具移動性、生成IAA 與ACC 脫氨酶的溶磷菌株，於甘藍菜、番茄、菠菜種子進行生物分析及三角瓶栽試驗，瞭解菌株對種子發芽與幼苗生長的影響，並以螢光原位雜合與純系分離株分子指紋分析確認接種菌株在植株根系的分布評估相容性，另於溫室中以盆栽試驗探討接種菌株對三種作物生長的影響並評估菌株在微棲地環境中的適應性。測試溶磷菌配合3 種作物的種子生物分析與三角瓶栽試驗，結果顯示其中2 株菌可提高瓶栽甘藍菜地上部乾重、4 株菌可提高瓶栽番茄地上部乾重、3 株菌可提高瓶栽菠菜地上部乾重，利用純系分離株BOX-PCR 分子指紋分析與16S rRNA 基因螢光探針EUB338 之雜合技術均顯示菌株於根系生長30 天後仍可存活於根表面。經相容性測試篩選出之菌株進行盆栽試驗，顯示可提高盆栽甘藍菜生質量具適應性之菌種為Bacillus marisflavi 與Pseudoarthrobacter niigatensis；可提高盆栽番茄生質量具適應性之菌種為Brevundimonas albigilva 與Gordonia hongkongensis；可提高盆栽菠菜生質量具適應性之菌種為Bacillus xiamenensis 與Paraburkholderia sacchari。
The present study was undertaken to study the colonizing behavior of phosphate solubilizing bacteria toward crops with high phosphorus demand. Motility, IAA and ACC deaminase producing abilities of phosphate solubilizing bacteria were screened first to obtain superior candidates. Bacterial inoculations were performed in seed bioassay and flask experiment. Considered for plant growth and bacterial colonization, the compatibilities of phosphate solubilizing bacteria toward cabbage, spinach and tomato were evaluated. The niche fitness of bacteria in soils will also be demonstrated in pot experiment. Out of the tested 35 strains were subjected to seed bioassay, and 8, 8, 3 strains were beneficial to seed germination of cabbage, tomato and spinach, respectively. Among them, 2 strains increased dry weight of above part of cabbage, 4 strains increased dry weight of above part of tomato, and 3 strains increased dry weight of above part of spinach. After 30 days of cultivation, inoculated strains still survived on root surface, as demonstrated using BOX-PCR fingerprinting of re-isolated strains and fluorescence in situ hybridization technique with EUB338 probe. Pot experiments showed that Bacillus marisflavi and Pseudoarthrobacter niigatensis can promote growth of cabbage, Brevundimonas albigilva and Gordonia hongkongensis can promote growth of tomato while Bacillus xiamenensis and Paraburkholderia sacchari can promote growth of spinach.
Event(s)
溶磷菌肥料肥(功)效評估及驗證研討會
Proceedings of the Symposium on Fertility and Function Assessment and its Verification for Phosphate-solubilizing Bacteria
Subjects
微生物肥料
溶磷菌
甘藍
番茄
菠菜
基因螢光探針
Microbial fertilizer
phosphate solubilizing bacteria
cabbage
tomato
spinach
gene fluorescent probe
Publisher
農業試驗所
Type
book part
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