|Title:||Herbaspirillum huttiensis為引起之薑黃葉桔病||Other Titles:||Occurrence of leaf blight of turmeric caused by Herbaspirillum huttiensis in Taiwan||Authors:||許秀惠
|Keywords:||薑黃;細菌性葉枯病;聚合酶連鎖反應;藥劑篩選;turmeric;bacterial leaf blight;PCR;agrochemical screening||Issue Date:||Mar-2009||Publisher:||中華民國植物病理學會||Journal Volume:||18||Journal Issue:||1||Start page/Pages:||13-22||Source:||植物病理學會刊||Abstract:||
2006年夏天，於南投縣名間及草屯等地區栽培之薑黃植株上發現由Acidovorax avenae subsp. avenae引起之薑黃葉枯病，同時發現另一病原菌可引起相同的病徵，該病原菌經生理生化特性確認屬於Burkholderia屬，再以對Burkholderia屬內不同的病原菌如B. andropogonis (引子對Pf/Pr)，B. caryophylli (引子對20L/20R nL/ nR)，B. cepacia (引子對CMG23-1/CM23-2)，B. gladioli (引子對CMG-23-1/G-23-2)及B. glumae (引子對1418S/1418A、1419S/1419A)具專一性之引子對進行PCR鑑定，結果均無反應，顯示供試病原菌與已知Burkholderia屬內的植物病原菌不同，經Biolog、脂肪酸分析及165 rDNA等分析結果顯示該病原菌為Herbaspirillum huttiensis。供試11種藥劑測試在一般使用濃度下對該病菌生長之抑制效果，顯示除鋅錳滅達樂對該病菌之生長無抑制作用外，其餘供試藥劑均能抑制此病原菌之生長，其中又以四環黴素之藥劑效果最佳，其效果依序為四環黴素、鏈四環黴素、嘉賜黴素、嘉賜銅、多保鏈黴素、鏈黴素、氫氧化銅、鹼性氯氧化銅、歐索林酸及三元硫酸銅。
Irregular browning lesion of the leaf margin was found on turmeric plants cultivated in Minjeng and Tstun of NanTou county in the summer of 2006 in Taiwan. Water-soaked lesions first appeared at leaf hydathodes and then spread along the leaf midrib. In later stage, lesions became darken and usually accompanied with yellowing halo and the color became darker. The same symptoms also appeared in the leaf sheath and finally the plant wilted. A gram-negative, rod-shaped bacterium was consistently isolated from the diseased tissues except Acidovorex avenae subsp. avenae. The unknown bacterium was identified as Herbaspirillum huttiensis based on its physiological and biochemical characteristics, the Biolog GN MicroPlate Identification System, fatty acid analysis, PCR, 16S rDNA and pathogenicity tests. According to the symptoms on the turmeric plants caused by H. huttiensis was also designed as bacterial blight of turmeric. In vitro screening for the efficacy of various agrochemicals to inhibit bacterial growth on NA plates showed that all tested chemicals except Ridomil MZ, including copper bactericides, antibiotics, oxolinic acid, and carbamates, were effective. Among them, tetracycline was the most effective.
|Appears in Collections:||植物保護系|
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