|Title:||伯利恆之星軟腐病之發生||Other Titles:||Occurrence of bacterial soft rot on Star of Bethleham||Authors:||許秀惠
|Keywords:||伯利恆之星;細菌性軟腐病;PCR;檢測;Star of Bethleham;bacterial soft rot disease;PCR;detection||Issue Date:||Jun-2008||Publisher:||中華民國植物病理學會||Journal Volume:||17||Journal Issue:||2||Start page/Pages:||177-182||Source:||植物病理學會刊||Abstract:||
於台中后里、嘉義新港、彰化田尾等地區栽培之伯利恆之星植株上發現組織水浸狀褐化軟腐的病徵，病徵通常從葉片基部或鱗莖開始發生，初呈水浸狀斑，迅速蔓延形成淡褐色或褐色或黑褐色軟腐，終至葉片或植植倒伏。經柯霍氏法則、生理生化、Biolog及PCR鑑定結果顯示分離所得之病原細菌包括Pectobacterium (原屬於Erwinia) carotovora subsp. carotovora (Ecc)及Pectobacterium (原屬於Erwinia) chrysanthemi (Ech)。調杳田間鱗莖及葉片組織軟腐病徵發生情形，發現不論鱗莖或葉片均以最外層（一～三層）組織最常發生軟腐病徵，其次為最內層組織，以Ec3F/Ec4R引子對檢測結果與觀察結果相同。以Ec3F/4R引子對檢測不同時期田間罹病植株的鱗華及葉片組織，結果顯示不論鱗莖或葉片組織於五月、六月及十二月份所取樣品均可檢測到Ecc及Ech二種軟腐病原，於二月、三月及四月所取樣品檢測結果均只檢測至Ecc一種軟腐病原，未測到Ech。
A soft-rot disease was found on the star of Bethleham plants cultivated in Taichung Howli, Chiayi Singang, Chunghua Tianwei areas. Symptoms were usually seen at the base of leaves or bulbs, beginning with water-soaking spots that developed into light brown or black brown soft-rot areas in late stage of infection. The diseased leaves or plants eventually fell down. Based on the physiological characteristics and the results from the Biolog GN MicroPlate system, polymerase chain reaction (PCR) and pathogenicity tests, bacteria isolated from the diseased tissues were identified as Pectobacterium (Erwinia) carotovora subsp. carotovora (Ecc) and Pectobacterium (Erwinia) chrysanthemi (Ech). Investigating the occurrence of soft-rot symptoms and PCR-detection using Ec3F/Ec4R primers on bulbs and leaf tissues in the fields showed that the outer layer tissue (layer 1~3) has the highest disease incidence, followed by the inner tissue. PCR detection with Ec3F/Ec4R primers of infected tissues at different time in the fields revealed that both Ecc and Ech could be detected in May, June, and December on bulbs and leaf tissues, whereas only Ecc, but not Ech, could be detected in the samples collected in February, March and April.
|Appears in Collections:||植物保護系|
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