Burkholderia andropogonis引起之檳榔葉斑病及藥劑篩選
Other Title
Occurrence of bacterial leaf spot of betel palm caused by Burkholderia andropogonis and inhibition of bacterial growth by agrochemicals
Journal
植物病理學會刊
Journal Volume
16
Journal Issue
3
Pages
131-139
Date Issued
2007-09
Date
2007-09
Author(s)
許秀惠
賴婉綺
潘雅碧
林俊義
Hseu, S.H.
Lai, W.C.
Pan, Y.P.
Lin, C.Y.
Abstract
民國95年間於台中縣大坑、嘉義縣阿里山鄉、屏東縣高樹鄉、高雄縣美濃鎮及花運縣鳳林鎮等地種植之檳榔葉片上發生褐化壞疽病斑，病斑周圍伴有黃暈，病斑隨著葉脈擴散呈不規則形，由柯霍氏法則、生理生化、Biolog鑑定及脂肪酸組成分析結果顯示供試檳榔病原菌株為Burkholderia andropogonis，進一步應用對B. andropogonis具有專一性之引子對進行PCR反應，結果由該病原菌可增幅出410 bP之專一性DNA條帶，並且以165 rDNA進行定序與鑑定，確認該病原菌為B. andropogonis，由其引起之病徵定名為檳榔葉斑病(bacterial leaf spot of betel palm)。
以市售12種藥劑測試在一般使用濃度下對該病菌生長之抑制效果，顯示12種藥劑均能抑制病原菌於PDA培養基上的生長，其中又以四環黴素效果最佳，依其抑制效果依序為四環黴素、歐索林酸、嘉賜黴素、嘉賜銅、氫氣化銅、鏈四環黴素、多保鏈黴素、三元硫酸銅、鋅錳乃浦、鏈黴素、鹼性氯氣化銅及鋅錳滅達樂等。
In 2006, a leaf spot disease was found on betel palm in several locations in Taiwan. The infected leaves showed irregular brown necrotic spots surrounded by yellowish halo. A gram-negative, rodshaped bacterium was consistently isolated from the diseased tissues. The bacterium was identified as Burkholderia andropogonis based on its physiological characteristics, the Biolog GN MicroPlate system, the MIS system for cellular fatty acid analysis, polymerase chain reaction (PCR) with a pair of primers specific for B. andropogonis, 16S rDNA sequence and pathogenicity tests. In vitro screening for the efficacy of different agrochemicals to inhibit bacterial growth on PDA plates showed that all tested chemicals, including copper bactericides, antibiotics, oxolinic acid, and carbamates, were effective. Among them, tetracycline was most effective.
Subjects
檳榔
葉斑病
藥劑篩選
betel palm
bacterial leaf spot
agrochemical screening
Publisher
中華民國植物病理學會
Type
journal article
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