近紅外光分析技術應用於糙米化學成分量之測定

Abstract

本硏究以近紅外光分析儀(near infrared reflectance spectroscopy, NIRS)進行稻糙米化學成份檢量線的建立,探討近紅外光分析術應用於糙米米粒化學成分測定的可行性。結果顯示NIRS所建立之水分,粗蛋白及直鏈澱粉等三條檢量如下:Msture(%)=20.21-36L67(1445nm)+213.31(1982nm)+143.75(2190nm)，相關係數(r)為0.99，成分範圍(CR)為8.65~15.46%；Crude protein(%)=4.28-2023,95(1722nm)+3664,6(1734nm)1776.03(1759nm)+68.01(1982nm)+468.35(227m)-395.65(2310nm),r=0•99,CR=5.84~10.77%;Amylose(%)=-194.96+8819,62(1445nm)-674,2(1940n-10136,5(2139nm)-6083.95(2208nm)+19560,3(227nm)-11191(28nm)，r=0.96，CR=3.5~31.16%。三條檢量線的相關係數均高達0,96以上；表示NIRS檢量線於糙米米粒化學成分測定上有相當的準確，可做發展此一技術供米質分析檢驗用。 The objectives of this study were to determine the moisture, crude protein and apparent amylose content by near infrared reflectance spectroscopy ( NIRS ) in brown rice. The results of calibration test as follows: Moisture(%)=20.21-361.67 (1445nm) + 213.31(1982nm) + 143.75(2190nm), correlation coefficient (r) =0.99 , content range(CR) -8.65~15.46% ; Crude protein(%) =4.28-2023.95(1722nm) +3664.62(1734nm)-1776.03(1759nm)+ 68.01(1982nm)+468.32(2270nm)-395.65 (2310nm) , r=0.99, CR:5.84~10.77; Amylose(%)=-194.96+8819.62(1445nm) - 674.2(1940nm) - 10136.5(2139nm) – 6083.95(2208nm)+19560.3(2270nm) - 11191 (2348nm), r=0.96, CR=3.5~31.16%. The correlation coefficients of the three regression equations were higher than 0.96. These results suggest that the MRS is a very useful tool to efficiently determine the three chemical consitutents rice quality research.