Cloning of Phytoene Desaturase Gene in Oncidium Gower Ramsey-Orchidaceae and Its Expression Analysis at Different Flower Stages
Journal
Journal International Cooperation
Journal Volume
7
Journal Issue
1
Pages
21-36
Date Issued
2012-03-13
Date
2012-03-13
Author(s)
Fure- Chyi Chen
Abstract
Oncidium Gower Ramsey is an important ornamental orchid in Taiwan. The bright yellow floral color is due to carotenoid accumulation in the flower tissues. In order to understand the expression of phytoene desaturase (PDS), a key enzyme for the biosynthesis of β-carotene, the cDNA encoding PDS was cloned and characterized during flower development. The full length OgrPDS cDNA contains 2,273 bp, including 1,593 bp open reading frame encoding for a polypeptide of 531 amino acid residues. The 5’ and 3’ untranslated regions (UTR) were 293 and 387 bp, respectively. The deduced amino acid sequence of this PDS has 72-75 % similarity to PDS from other plant species. The relative expression level of the OgrPDS in roots and leaves was investigated. The result showed that the expression level of OgrPDS at different flower developmental stages increased gradually until stage 5 which two- and three- fold higher than leaves and roots, respectively.
Subjects
gene expression
phytoene desaturase
Oncidium Gower Ramsey
carotenoid
Publisher
International Cooperation and Development Fund
Type
other