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  1. DSpace-CRIS at My University
  2. 一、研究單位
  3. 鳳山分所
  4. 熱帶果樹系
Please use this identifier to cite or link to this item: https://scholars.tari.gov.tw/handle/123456789/9255
Title: Ectopic Expression of Oncidium Gower Ramsey Phytoene Synthase Gene Caused Pale Flower and Reduced Leaf Size in Transgenic Tobacco
Authors: Wen-Li Lee 
Jian-Zhi Huang
Fure-Chyi Chen
Keywords: carotenoids;ectopic expression;gene expression;Oncidium Gower Ramsey;phytoene synthases
Issue Date: 18-Sep-2012
Publisher: International Cooperation and Development Fund
Journal Volume: 7
Journal Issue: 2
Start page/Pages: 133-150
Source: Journal International Cooperation 
Abstract: 
Oncidium Gower Ramsey is an important orchid for cut flower production and
export in Taiwan. The bright yellow flower color of Oncidium Gower Ramsey is due to
the accumulation of carotenoids. The phytoene synthase (PSY) gene, a key enzyme for
the biosynthesis of carotenoids was cloned, by isolating total RNA from labellum and
reverse transcription followed by polymerse chain reaction. A full-length sequence of the
Oncidium PSY cDNA (OncPSY) was obtained by rapid amplification of cDNA ends
(RACE). Phylogenetic analysis revealed evolutionary conservation of the encoding
OncPSY protein sequence. RT-PCR analysis showed that OncPSY was highly expressed
in flowers and leaves, but less in roots. Ectopic expression of 35S::OncPSY in tobacco
plant resulted in severe phenotypic effects including dwarfing, altered leaf morphology,
and reduced flower color.
URI: https://scholars.tari.gov.tw/handle/123456789/9255
https://jic.npust.edu.tw/news/news.aspx
ISSN: 1992-1497
Appears in Collections:熱帶果樹系

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